Exploring the Educational Potential of Mutualistic Interaction between Cynometra cauliflora and Arbuscular Mycorrhiza in PT Chandra Asri’s Biodiversity Park: A Content Analysis for High School Biology Learning

Najmi Firdaus, Dian Rachmawati, Rida Oktorida Khastini, Siti Gia Syauqiyah Fitri, Evi Amelia, Desi Eka Nur Fitriana, Rifki Survani, Indria Wahyuni

Abstract


Arbuscular mycorrhizal fungi (AMF) form mutualistic interactions with plants, including Cynometra cauliflora (Namnam), a tropical indigenous species from the Fabaceae family. This study explores the educational potential of this biological relationship to enrich high school biology education under the Kurikulum Merdeka framework. Field research was conducted at PT Chandra Asri’s Biodiversity Park in Cilegon, Banten Province, Indonesia, an area with rich local biodiversity and a tropical rainforest climate.  The study used a qualitative content analysis approach following an exploratory-descriptive model. Nine naturally growing C. cauliflora individuals were selected for morphological observations and root sampling. Morphological characteristics were documented following standard botanical protocols, while AMF colonization was assessed through root clearing, Phillips and Hayman staining method, and microscopic observation. The percentage of AMF colonization was calculated using the gridline intersect method. Habitat characterization to understand the ecological context. Curriculum analysis reviewed Indonesian biology standards, textbooks, and scientific literature to map potential integrations. Key concepts such as mutualism, nutrient cycling, biodiversity conservation, and plant physiology were identified for curriculum enrichment. The findings suggest that studying C. cauliflora and its AMF association can effectively support Capaian Pembelajaran (Learning Achievement Targets), fostering student skills in scientific methods, environmental awareness, and critical thinking. Incorporating local examples promotes contextual and inquiry-based learning, aligning with the spirit of the Kurikulum Merdeka and strengthening students’ ecological literacy. In conclusion, the mutualistic relationship between C. cauliflora and AMF offers a valuable, locally relevant scientific model for enhancing biology education. It bridges scientific knowledge, cultural understanding, and sustainability, preparing students for real-world ecological challenges.


Keywords


Arbuscular mycorrhyza, biology subject, Namnam, Taman Kehati

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Ado, M. A., Abas, F., Ismail, I. S., Ghazali, H. M., & Shaari, K. (2015). Chemical profile and antiacetylcholinesterase, antityrosinase, antioxidant and α-glucosidase inhibitory activity of Cynometra cauliflora L. leaves. Journal of the Science of Food and Agriculture, 95(3), 635–642. https://doi.org/10.1002/jsfa.6832

Allen, D., & Tanner, K. (2005). Approaches to biology teaching and learning: From a scholarly approach to teaching to the scholarship of teaching. Cell Biology Education, 4(1), 1–6. https://doi.org/10.1187/cbe.04-11-0052

Birhane, E., Gebretsadik, K. F., Taye, G., Aynekulu, E., Rannestad, M. M., & Norgrove, L. (2020). Effects of forest composition and disturbance on arbuscular mycorrhizae spore density, arbuscular mycorrhizae root colonization, and soil carbon stocks in a dry afromontane forest in northern Ethiopia. Diversity, 12(4), 133. https://doi.org/10.3390/d12040133

Boyer Commission on Educating Undergraduates in the Research University. (1998). Reinventing undergraduate education: A blueprint for America's research universities. State University of New York at Stony Brook for the Carnegie Foundation for the Advancement of Teaching.

Buerkle, A., O'Dell, A., Matharu, H., Buerkle, L., & Ferreira, P. (2023). Recommendations to align higher education teaching with the UN sustainability goals – A scoping survey. International Journal of Educational Research Open, 5, 100280. https://doi.org/10.1016/j.ijedro.2023.100280

Carter, N., Bryant-Lukosius, D., DiCenso, A., Blythe, J., & Neville, A. J. (2014). The use of triangulation in qualitative research. Oncology Nursing Forum, 41(5), 545–547. https://doi.org/10.1188/14.ONF.545-547

Chiu, C., & Paszkowski, U. (2019). Mechanisms and impact of symbiotic phosphate acquisition. Cold Spring Harbor Perspectives in Biology, 11(6), a034603. https://doi.org/10.1101/cshperspect.a034603

Darling-Hammond, L., Flook, L., Cook-Harvey, C., Barron, B., & Osher, D. (2019). Implications for educational practice of the science of learning and development. Applied Developmental Science, 24(2), 97–140. https://doi.org/10.1080/10888691.2018.1537791

Das, S., & Sarkar, S. (2024). Arbuscular mycorrhizal fungal contribution towards plant resilience to drought conditions. Frontiers in fungal biology, 5, 1355999. https://doi.org/10.3389/ffunb.2024.1355999

Datumaya, S. C. P., & Angio, M. H. (2023). Morphology and study of local fruit potential of Namnam (Cynometra cauliflora) collection of Purwodadi Botanical Garden, East Java. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia, 9, 153–157.

Dibner, K. A., & Snow, C. E. (Eds.). (2016). Science literacy: Concepts, contexts, and consequences. National Academies Press. https://doi.org/10.17226/23595

Erheij, E. W. M., & Coronel, R. E. (1997). Prosea: Sumber daya nabati Asia Tenggara, 2. Buah-buahan yang dapat dimakan [Indonesian]. Gramedia Pustaka Utama.

Felder, R. M. (1997). Beating the numbers game: Effective teaching in large classes. Paper presented at the ASEE Annual Conference, Milwaukee, WI. http://ncsu.edu/felder-public/Papers/Largeclasses.htm

Giovannetti, M., & Mosse, B. (1980). An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytologist, 84, 489–500. https://doi.org/10.1111/j.1469-8137.1980.tb04556.x

Han, S., Wang, X., Cheng, Y., Wu, G., Dong, X., He, X., & Zhao, G. (2023). Multidimensional analysis reveals environmental factors that affect community dynamics of arbuscular mycorrhizal fungi in poplar roots. Frontiers in Plant Science, 13, https://doi.org/10.3389/fpls.2022.1068527

Handelsman, J., Ebert-May, D., Beichner, R., Bruns, P., Chang, A., DeHaan, R., Gentile, J., Lauffer, S., Stewart, J., Tilghman, S. M., & Wood, W. B. (2004). Scientific teaching. Science, 304(5670), 521–522. https://doi.org/10.1126/science.1096022

Hester, S. D., Nadler, M., Katcher, J., Elfring, L. K., Dykstra, E., Rezende, L. F., & Bolger, M. S. (2018). Authentic Inquiry through Modeling in Biology (AIM-Bio): An introductory laboratory curriculum that increases undergraduates' scientific agency and skills. CBE—Life Sciences Education, 17(4), ar63. https://doi.org/10.1187/cbe.18-06-0090

Hewedy, O. A., Elsheery, N. I., Karkour, A. M., Elhamouly, N., Arafa, R. A., Mahmoud, G. A. E., ... & Brestic, M. (2023). Jasmonic acid regulates plant development and orchestrates stress response during tough times. Environmental and Experimental Botany, 208, 105260.

Heyne, K. (1987). Tumbuhan berguna Indonesia (Vol. 2). Sarana Wana Jaya. [Indonesian]

Hong, P., Schmid, B., De Laender, F., Eisenhauer, N., Zhang, X., Chen, H., Craven, D., De Boeck, H. J., Hautier, Y., Petchey, O. L., Reich, P. B., Steudel, B., Striebel, M., Thakur, M. P., & Wang, S. (2022). Biodiversity promotes ecosystem functioning despite environmental change. Ecology Letters, 25(2), 555–569. https://doi.org/10.1111/ele.13936

Hovland, M., Mata-González, R., Schreiner, R. P., & Rodhouse, T. J. (2019). Fungal facilitation in rangelands: Do arbuscular mycorrhizal fungi mediate resilience and resistance in sagebrush steppe? Rangeland Ecology & Management, 72(4), 678–691. https://doi.org/10.1016/j.rama.2019.02.004

Ilyas, U., du Toit, L. J., Hajibabaei, M., & McDonald, M. R. (2024). Influence of plant species, mycorrhizal inoculant, and soil phosphorus level on arbuscular mycorrhizal communities in onion and carrot roots. Frontiers in Plant Science, 14, 1324626. https://doi.org/10.3389/fpls.2023.1324626

Jeffs, L., McShane, J., Flintoft, V., White, P., Indar, A., Maione, M., Lopez, A. J., Bookey-Bassett, S., & Scavuzzo, L. (2016). Contextualizing learning to improve care using collaborative communities of practices. BMC health services research, 16(1), 464. https://doi.org/10.1186/s12913-016-1566-4

Khaliq, A., Perveen, S., Alamer, K. H., Zia Ul Haq, M., Rafique, Z., Alsudays, I. M., Althobaiti, A. T., Saleh, M. A., Hussain, S., & Attia, H. (2022). Arbuscular mycorrhizal fungi symbiosis to enhance plant–soil interaction. Sustainability, 14(13), 7840. https://doi.org/10.3390/su14137840

Khastini, R., Lestari, I. D., Sari, I. J., & Septiani, N. Exploring the Diversity of Arbuscular Mycorrhizal Fungi in Zingiberaceae Family Plants at the Tukung Gede Mountain Natural Reserve. Journal of Tropical Biodiversity and Biotechnology, 10(1), jtbb12567-jtbb12567. https://doi.org/10.22146/jtbb.12567

Kong, Y. (2021). The role of experiential learning on students' motivation and classroom engagement. Frontiers in Psychology, 12, 771272. https://doi.org/10.3389/fpsyg.2021.771272

Lattimer, H., & Riordan, R. (2011). Project-based learning engages students in meaningful work: Students at High Tech Middle engage in project-based learning. Middle School Journal, 43(2), 18–23. https://doi.org/10.2307/23024494

Na-Ah, R. F., Ngwa, N. N., Tandzi, L. N., Tchatchouang, E. N., Zerpa-Catanho, D. P., Youmbi, E., & Tonfack, L. B. (2024). Seed germination, morphology and fruit phenology insight of Cylicomorpha solmsii (Urb.) Urb: A step towards sustainable restoration planning. Scientific Reports, 14(1), 16995. https://doi.org/10.1038/s41598-024-66018-9

National Research Council. (1997). Science teaching reconsidered: A handbook. National Academies Press.

National Research Council. (2003). Improving undergraduate instruction in science, technology, engineering, and mathematics: Report of a workshop. National Academies Press. https://doi.org/10.17226/10711

National Science Foundation. (1996). Shaping the future: New experiences for undergraduate education in science, mathematics, engineering, and technology. National Science Foundation.

Oliveira, A. P., Bajanca, A., & Paramés, A. (2025). Unveiling urban biodiversity: An interdisciplinary hands-on project that catalyzes awareness. Environmental and Sustainability Indicators, 25, 100561. https://doi.org/10.1016/j.indic.2024.100561

Qi, S., Wang, J., Wan, L., Dai, Z., Matos, D. M. da S., Du, D., Egan, S., Bonser, S. P., Thomas, T., & Moles, A. T. (2022). Arbuscular mycorrhizal fungi contribute to phosphorus uptake and allocation strategies of Solidago canadensis in a phosphorus-deficient environment. Frontiers in Plant Science, 13, 831654. https://doi.org/10.3389/fpls.2022.831654

Renjana, E., Firdiana, E. R., Angio, M. H., Ningrum, L. W., Lailaty, I. Q., Rahadiantoro, A., Martiansyah, I., Zulkarnaen, R., Rahayu, A., Raharjo, P. D., Abywijaya, I. K., Usmadi, D., Risna, R. A., Cropper, W. P., & Yudaputra, A. (2024). Spatial habitat suitability prediction of essential oil wild plants on Indonesia’s degraded lands. PeerJ, 12, e17210. https://doi.org/10.7717/peerj.17210

Rillig, M. C., & Mummey, D. L. (2006). Mycorrhiza and soil structure. New Phytologist, 171(1), 41–53. https://doi.org/10.1111/j.1469-8137.2006.01750.x

Samling, B. A., Assim, Z., Tong, W.-Y., Leong, C.-R., Ab Rashid, S., Nik Mohamed Kamal, N. N. S., Muhamad, M., & Tan, W.-N. (2021). Cynometra cauliflora L.: An indigenous tropical fruit tree in Malaysia bearing essential oils and their biological activities. Arabian Journal of Chemistry, 14(9), 103302. https://doi.org/10.1016/j.arabjc.2021.103302

Setiani, D. E., Rahmawati, I., Primandiri, P. R., & Santoso, A. M. (2022). Karakterisasi morfologi tanaman Namnam (Cynometra cauliflora) di Kabupaten Kediri. Prosiding Seminar Nasional Kesehatan, Sains dan Pembelajaran, 2(1). https://proceeding.unpkediri.ac.id/index.php/seinkesjar/article/view/3063/2132

Setyaningsih, L. (2023). Ketergantungan semai johar (Cassia siamea Lamk) terhadap fungi mikoriza arbuskula pada media tailing tambang emas. Makila, 17(2), 238–250. https://doi.org/10.30598/makila.v17i2.11010

Smith, K. A., Sheppard, S. D., Johnson, D. W., & Johnson, R. T. (2005). Pedagogies of engagement: Classroom-based practices. Journal of Engineering Education, 94(1), 87–101. https://doi.org/10.1002/j.2168-9830.2005.tb00831.x

Sujarwo, S., Oktaviana, R., Bina, U., & Palembang, D. (2017). Pengaruh warna terhadap short term memory pada siswa kelas VIII SMPN 37 Palembang. Psikis: Jurnal Psikologi Islami, 3(1), 33–42. https://doi.org/10.19109/psikis.v3i1.1391

Suprapto, P. K., Ardiansyah, R., Diella, D., Chaidir, D. M., Hendrawan, A. M., Diana, S. W., & Amarulloh, A. (2024). Plant anatomy: Definition, classification of plant organs and tissue, and design of a visuospatial transformation learning model for teaching biology. Indonesian Journal of Science and Technology, 9(2), 557–584. https://doi.org/10.17509/ijost.v9i2.72105

Susiyawati, E., & Treagust, D. F. (2021). Students’ visual literacy: A study from plant anatomy learning. Journal of Physics: Conference Series, 1747(1), 012021. https://doi.org/10.1088/1742-6596/1747/1/012021

Tajudin, T. J., Mat, N., Siti-Aishah, A. B., Yusran, A. A., Alwi, A., & Ali, A. M. (2012). Cytotoxicity, antiproliferative effects, and apoptosis induction of methanolic extract of Cynometra cauliflora Linn. whole fruit on human promyelocytic leukemia HL-60 cells. Evidence-Based Complementary and Alternative Medicine, 2012, 127373. https://doi.org/10.1155/2012/127373

Verheij, E. W. M., & Coronel, R. E. (1997). Southeast Asian Vegetable Resources 2: Edible Fruits. Jakarta (ID): Gramedia Main Library.

Vydra, M., & Kováčik, J. (2024). Visual plant anatomy: From science to education and vice versa. Journal of Biosciences, 49(2), 1–10. https://doi.org/10.1007/s12038-024-00436-9

Wahab, A., Muhammad, M., Munir, A., Abdi, G., Zaman, W., Ayaz, A., Khizar, C., & Reddy, S. P. P. (2023). Role of arbuscular mycorrhizal fungi in regulating growth, enhancing productivity, and potentially influencing ecosystems under abiotic and biotic stresses. Plants, 12(17), 3102. https://doi.org/10.3390/plants12173102

Wu, Y., Chen, C., & Wang, G. (2024). Inoculation with arbuscular mycorrhizal fungi improves plant biomass and nitrogen and phosphorus nutrients: A meta-analysis. BMC Plant Biology, 24, 960. https://doi.org/10.1186/s12870-024-05638-9

Yang, L., Qi, B., & Guo, Q. (2024). The effect of icon color combinations in information interfaces on task performance under varying levels of cognitive load. Applied Sciences, 14(10), 4212. https://doi.org/10.3390/app14104212




DOI: https://doi.org/10.53889/ijbetsd.v5i1.636

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